› 1ntz

X-ray diffraction
2.6Å resolution

Crystal Structure of Mitochondrial Cytochrome bc1 Complex Bound with Ubiquinone

Released:
DOI: 10.2210/pdb1ntz/pdb
PDB entry 1ntz coloured by chain, front view.
PDB entry 1ntz coloured by chain, side view.
PDB entry 1ntz coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis for the quinone reduction in the bc1 complex: a comparative analysis of crystal structures of mitochondrial cytochrome bc1 with bound substrate and inhibitors at the Qi site.
Gao X, Wen X, Esser L, Quinn B, Yu L, Yu CA, Xia D
Biochemistry 42 9067-80 (2003)
PMID: 12885240

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132263 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 446 amino acids
Theoretical weight: 49.27 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P31800 (Residues: 35-480; Coverage: 93%)
Gene name: UQCRC1
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Cytochrome b-c1 complex subunit 2, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 439 amino acids
Theoretical weight: 46.58 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P23004 (Residues: 15-453; Coverage: 97%)
Gene name: UQCRC2
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Cytochrome b Chain: C
Molecule details ›
Chain: C
Length: 379 amino acids
Theoretical weight: 42.62 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00157 (Residues: 1-379; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1, heme protein, mitochondrial Chain: D
Molecule details ›
Chain: D
Length: 241 amino acids
Theoretical weight: 27.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00125 (Residues: 85-325; Coverage: 74%)
Gene name: CYC1
Sequence domains: Cytochrome C1 family
Structure domains:
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chain: E
Molecule details ›
Chain: E
Length: 196 amino acids
Theoretical weight: 21.64 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13272 (Residues: 79-274; Coverage: 72%)
Gene name: UQCRFS1
Sequence domains:
Structure domains:
Cytochrome b-c1 complex subunit 7 Chain: F
Molecule details ›
Chain: F
Length: 110 amino acids
Theoretical weight: 13.37 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00129 (Residues: 2-111; Coverage: 99%)
Gene name: UQCRB
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Structure domains: Cytochrome b-c1 complex subunit 7
Cytochrome b-c1 complex subunit 8 Chain: G
Molecule details ›
Chain: G
Length: 81 amino acids
Theoretical weight: 9.61 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13271 (Residues: 2-82; Coverage: 99%)
Gene name: UQCRQ
Sequence domains: UcrQ family
Structure domains: Cytochrome b-c1 complex subunit 8
Cytochrome b-c1 complex subunit 6, mitochondrial Chain: H
Molecule details ›
Chain: H
Length: 78 amino acids
Theoretical weight: 9.19 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00126 (Residues: 14-91; Coverage: 86%)
Gene name: UQCRH
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Structure domains: Ubiquinol-cytochrome C reductase hinge domain
Cytochrome b-c1 complex subunit 9 Chain: I
Molecule details ›
Chain: I
Length: 57 amino acids
Theoretical weight: 5.82 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13272 (Residues: 1-57; Coverage: 21%)
Gene name: UQCRFS1
Sequence domains: Ubiquinol-cytochrome c reductase 8 kDa, N-terminal
Structure domains: Cytochrome Bc1 Complex; Chain I
Cytochrome b-c1 complex subunit 9 Chain: J
Molecule details ›
Chain: J
Length: 62 amino acids
Theoretical weight: 7.21 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00130 (Residues: 2-63; Coverage: 97%)
Gene name: UQCR10
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Structure domains: Cytochrome b-c1 complex subunit 9
Cytochrome b-c1 complex subunit 10 Chain: K
Molecule details ›
Chain: K
Length: 56 amino acids
Theoretical weight: 6.57 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P07552 (Residues: 1-56; Coverage: 100%)
Gene names: UQCR, UQCR11
Sequence domains: Ubiquinol-cytochrome C reductase complex, 6.4kD protein
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments


Cofactor: Ligand UQ2 2 x UQ2

Cofactor: Ligand HEM 3 x HEM
1 bound ligand:
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: I4122
Unit cell:
a: 153.828Å b: 153.828Å c: 596.671Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.283

Quick links

Citations

23 review citations

The cytochrome bc1 complex: function in the context of structure.
Crofts AR. (2004)
22 more

19 mentions without citation

The Q cycle of cytochrome bc complexes: a structure perspective.
Cramer et al. (2011)
18 more