› 1umk

X-ray diffraction
1.75Å resolution

The Structure of Human Erythrocyte NADH-cytochrome b5 Reductase

Released:
DOI: 10.2210/pdb1umk/pdb
PDB entry 1umk coloured by chain, front view.
PDB entry 1umk coloured by chain, side view.
PDB entry 1umk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human erythrocyte NADH-cytochrome b5 reductase.
Bando S, Takano T, Yubisui T, Shirabe K, Takeshita M, Nakagawa A
Acta Crystallogr D Biol Crystallogr 60 1929-34 (2004)
PMID: 15502298

Function and Biology Details

Reaction catalysed: 
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH-cytochrome b5 reductase 3 Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00387 (Residues: 27-301; Coverage: 91%)
Gene names: CYB5R3, DIA1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P41
Unit cell:
a: 65.869Å b: 65.869Å c: 76.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.165 0.207
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Recessive congenital methaemoglobinaemia: cytochrome b(5) reductase deficiency.
Percy et al. (2008)
4 more

22 mentions without citation

Structural Features of Cytochrome b5-Cytochrome b5 Reductase Complex Formation and Implications for the Intramolecular Dynamics of Cytochrome b5 Reductase.
Gutiérrez-Merino et al. (2021)
21 more