› 1w4w

X-ray diffraction
1.55Å resolution

Ferric horseradish peroxidase C1A in complex with formate

Released:
DOI: 10.2210/pdb1w4w/pdb
PDB entry 1w4w coloured by chain, front view.
PDB entry 1w4w coloured by chain, side view.
PDB entry 1w4w coloured by chain, top view.
Source organism: Armoracia rusticana
Primary publication:
Complexes of horseradish peroxidase with formate, acetate, and carbon monoxide.
Carlsson GH, Nicholls P, Svistunenko D, Berglund GI, Hajdu J
Biochemistry 44 635-42 (2005)
PMID: 15641789

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132478 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxidase C1A Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.65 KDa
Source organism: Armoracia rusticana
Expression system: Escherichia coli
UniProt:
  • Canonical: P00433 (Residues: 31-353; Coverage: 100%)
Gene names: HPRC1, PRXC1A
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand CA 2 x CA
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 40.33Å b: 68.302Å c: 117.048Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.215
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Functional aspects of protein flexibility.
Teilum et al. (2009)
6 more

6 mentions without citation

A scalable serology solution for profiling humoral immune responses to SARS-CoV-2 infection and vaccination.
Colwill et al. (2022)
5 more