› 1xw7

X-ray diffraction
2.3Å resolution

Diabetes-Associated Mutations in Human Insulin: Crystal Structure and Photo-Cross-Linking Studies of A-Chain Variant Insulin Wakayama

Released:
DOI: 10.2210/pdb1xw7/pdb
PDB entry 1xw7 coloured by chain, front view.
PDB entry 1xw7 coloured by chain, side view.
PDB entry 1xw7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Diabetes-associated mutations in human insulin: crystal structure and photo-cross-linking studies of a-chain variant insulin Wakayama.
Wan ZL, Huang K, Xu B, Hu SQ, Wang S, Chu YC, Katsoyannis PG, Weiss MA
Biochemistry 44 5000-16 (2005)
PMID: 15794638

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-134224 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

3 bound ligands:
Ligand IPH 2 x IPH
Ligand ZN 2 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER
Spacegroup: P213
Unit cell:
a: 70.852Å b: 70.852Å c: 70.852Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.269
Expression system: Not provided

Quick links

Citations

9 review citations

Insulin receptor isoforms and insulin receptor/insulin-like growth factor receptor hybrids in physiology and disease.
Belfiore et al. (2009)
8 more

2 mentions without citation

Understanding insulin and its receptor from their three-dimensional structures.
Lawrence MC. (2021)
1 more