› 1a4x

X-ray diffraction
2.3Å resolution

PYRR, THE BACILLUS SUBTILIS PYRIMIDINE BIOSYNTHETIC OPERON REPRESSOR, HEXAMERIC FORM

Released:
DOI: 10.2210/pdb1a4x/pdb
PDB entry 1a4x coloured by chain, front view.
PDB entry 1a4x coloured by chain, side view.
PDB entry 1a4x coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Adaptation of an enzyme to regulatory function: structure of Bacillus subtilis PyrR, a pyr RNA-binding attenuation protein and uracil phosphoribosyltransferase.
Tomchick DR, Turner RJ, Switzer RL, Smith JL
Structure 6 337-50 (1998)
PMID: 9551555

Function and Biology Details

Reaction catalysed: 
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153943 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein PyrR Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 20.29 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P39765 (Residues: 1-181; Coverage: 100%)
Gene names: BSU15470, pyrR
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: R32
Unit cell:
a: 100.58Å b: 100.58Å c: 275.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.247
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors.
Turnbough et al. (2008)
8 more

2 mentions without citation

Repression of the pyr operon in Lactobacillus plantarum prevents its ability to grow at low carbon dioxide levels.
Nicoloff et al. (2005)
1 more