› 1exn

X-ray diffraction
2.5Å resolution

T5 5'-EXONUCLEASE

Released:
DOI: 10.2210/pdb1exn/pdb
PDB entry 1exn coloured by chain, front view.
PDB entry 1exn coloured by chain, side view.
PDB entry 1exn coloured by chain, top view.
Source organism: Escherichia virus T5
Primary publication:
A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease.
Ceska TA, Sayers JR, Stier G, Suck D
Nature 382 90-3 (1996)
PMID: 8657312

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flap endonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 33.6 KDa
Source organism: Escherichia virus T5
Expression system: Escherichia coli
UniProt:
  • Canonical: P06229 (Residues: 2-291; Coverage: 100%)
Gene names: D15, T5.130, T5p128, exo5
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BL19
Spacegroup: P43
Unit cell:
a: 77.76Å b: 77.76Å c: 134.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.339
Expression system: Escherichia coli

Quick links

Citations

21 review citations

The FEN-1 family of structure-specific nucleases in eukaryotic DNA replication, recombination and repair.
Lieber MR. (1997)
20 more

11 mentions without citation

Topoisomerases and site-specific recombinases: similarities in structure and mechanism.
Yang W. (2010)
10 more