› 1gpf

X-ray diffraction
1.85Å resolution

CHITINASE B FROM SERRATIA MARCESCENS IN COMPLEX WITH INHIBITOR PSAMMAPLIN

Released:
DOI: 10.2210/pdb1gpf/pdb
PDB entry 1gpf coloured by chain, front view.
PDB entry 1gpf coloured by chain, side view.
PDB entry 1gpf coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Psammaplin A, a chitinase inhibitor isolated from the Fijian marine sponge Aplysinella rhax.
Tabudravu JN, Eijsink VG, Gooday GW, Jaspars M, Komander D, Legg M, Synstad B, van Aalten DM
Bioorg Med Chem 10 1123-8 (2002)
PMID: 11836123

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH18 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 55.52 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54276 (Residues: 1-499; Coverage: 100%)
Gene name: chiB
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 55.754Å b: 103.588Å c: 186.065Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.245
Expression system: Escherichia coli

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Citations

15 review citations

Biotechnological aspects of chitinolytic enzymes: a review.
Dahiya et al. (2006)
14 more