› 1k03

X-ray diffraction
2.7Å resolution

Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn Complexed with Para-hydroxy Benzaldehyde

Released:
DOI: 10.2210/pdb1k03/pdb
PDB entry 1k03 coloured by chain, front view.
PDB entry 1k03 coloured by chain, side view.
PDB entry 1k03 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The role of glutamine 114 in old yellow enzyme.
Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V
J Biol Chem 277 2138-45 (2002)
PMID: 11668181

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase 1 Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 44.93 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02899 (Residues: 2-400; Coverage: 100%)
Gene name: OYE1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
Ligand MG 1 x MG
Ligand HBA 1 x HBA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43212
Unit cell:
a: 143Å b: 143Å c: 43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 0.13 not available
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Applications of protein engineering to members of the old yellow enzyme family.
Amato et al. (2015)
14 other articles

3 mentions without citation

Crystal structure of New Delhi metallo-β-lactamase reveals molecular basis for antibiotic resistance.
King et al. (2011)
2 more