› 1lxm

X-ray diffraction
2.2Å resolution

Crystal Structure of Streptococcus agalactiae Hyaluronate Lyase Complexed with Hexasaccharide Unit of Hyaluronan

Released:
DOI: 10.2210/pdb1lxm/pdb
PDB entry 1lxm coloured by chain, front view.
PDB entry 1lxm coloured by chain, side view.
PDB entry 1lxm coloured by chain, top view.
Source organism: Streptococcus agalactiae
Primary publication:
Structure and flexibility of Streptococcus agalactiae hyaluronate lyase complex with its substrate. Insights into the mechanism of processive degradation of hyaluronan.
Mello LV, De Groot BL, Li S, Jedrzejas MJ
J Biol Chem 277 36678-88 (2002)
PMID: 12130645

Function and Biology Details

Reaction catalysed: 
Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176060 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BDP
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 51.108Å b: 155.043Å c: 238.136Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.271
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Hyaluronidases: their genomics, structures, and mechanisms of action.
Stern et al. (2006)
3 more

6 mentions without citation

Atomic-Resolution Experimental Structural Biology and Molecular Dynamics Simulations of Hyaluronan and Its Complexes.
Guvench O. (2022)
5 more