› 1nek

X-ray diffraction
2.6Å resolution

Complex II (Succinate Dehydrogenase) From E. Coli with ubiquinone bound

Released:
DOI: 10.2210/pdb1nek/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1nek coloured by chain, front view.
PDB entry 1nek coloured by chain, side view.
PDB entry 1nek coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Architecture of succinate dehydrogenase and reactive oxygen species generation.
Yankovskaya V, Horsefield R, Törnroth S, Luna-Chavez C, Miyoshi H, Léger C, Byrne B, Cecchini G, Iwata S
Science 299 700-4 (2003)
PMID: 12560550

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero dodecamer
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-139344 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Succinate dehydrogenase flavoprotein subunit Chain: A
Molecule details ›
Chain: A
Length: 588 amino acids
Theoretical weight: 64.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC41 (Residues: 1-588; Coverage: 100%)
Gene names: JW0713, b0723, sdhA
Sequence domains:
Structure domains:
Succinate dehydrogenase iron-sulfur subunit Chain: B
Molecule details ›
Chain: B
Length: 238 amino acids
Theoretical weight: 26.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07014 (Residues: 1-238; Coverage: 100%)
Gene names: JW0714, b0724, sdhB
Sequence domains:
Structure domains:
Succinate dehydrogenase cytochrome b556 subunit Chain: C
Succinate dehydrogenase hydrophobic membrane anchor subunit Chain: D

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand UQ2 1 x UQ2

Cofactor: Ligand HEM 1 x HEM
7 bound ligands:
Ligand OAA 1 x OAA
Ligand CA 2 x CA
Ligand FES 1 x FES
Ligand SF4 1 x SF4
Ligand F3S 1 x F3S
Ligand CDN 1 x CDN
Ligand EPH 1 x EPH
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 138.8Å b: 138.8Å c: 521.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.247 0.289
Expression system: Escherichia coli

Quick links

Citations

129 review citations

Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release.
Zorov et al. (2014)
128 more

51 mentions without citation

Distribution and dynamics of electron transport complexes in cyanobacterial thylakoid membranes.
Liu LN. (2016)
50 more