› 1qs2

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF VIP2 WITH NAD

Released:
DOI: 10.2210/pdb1qs2/pdb
PDB entry 1qs2 coloured by chain, front view.
PDB entry 1qs2 coloured by chain, side view.
PDB entry 1qs2 coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Evolution and mechanism from structures of an ADP-ribosylating toxin and NAD complex.
Han S, Craig JA, Putnam CD, Carozzi NB, Tainer JA
Nat Struct Biol 6 932-6 (1999)
PMID: 10504727

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP ribosyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 401 amino acids
Theoretical weight: 45.56 KDa
Source organism: Bacillus cereus
Expression system: Bacillus cereus
UniProt:
  • Canonical: Q844J9 (Residues: 62-462; Coverage: 87%)
Gene names: vip2Ac, vip2Ac2
Sequence domains: ADP-ribosyltransferase exoenzyme
Structure domains: Toxin ADP-ribosyltransferase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 53.673Å b: 70.564Å c: 142.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.266
Expression system: Bacillus cereus

Quick links

Citations

35 review citations

Toward a unified nomenclature for mammalian ADP-ribosyltransferases.
Hottiger et al. (2010)
34 more

8 mentions without citation

Insecticidal Activity of Bacillus thuringiensis Proteins Against Coleopteran Pests.
Domínguez-Arrizabalaga et al. (2020)
7 more