› 1r2j

X-ray diffraction
2.1Å resolution

FkbI for Biosynthesis of Methoxymalonyl Extender Unit of Fk520 Polyketide Immunosuppresant

Released:
DOI: 10.2210/pdb1r2j/pdb
PDB entry 1r2j coloured by chain, front view.
PDB entry 1r2j coloured by chain, side view.
PDB entry 1r2j coloured by chain, top view.
Source organism: Streptomyces hygroscopicus
Primary publication:
Crystal structure of an Acyl-ACP dehydrogenase from the FK520 polyketide biosynthetic pathway: insights into extender unit biosynthesis.
Watanabe K, Khosla C, Stroud RM, Tsai SC
J Mol Biol 334 435-44 (2003)
PMID: 14623185

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FkbI Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 38.23 KDa
Source organism: Streptomyces hygroscopicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KIE5 (Residues: 1-366; Coverage: 100%)
Gene name: fkbI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 1 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I41
Unit cell:
a: 116.55Å b: 116.55Å c: 126.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.263
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Biosynthesis of polyketide synthase extender units.
Chan et al. (2009)
5 more

2 mentions without citation

Self-association of a highly charged arginine-rich cell-penetrating peptide.
Tesei et al. (2017)
1 more