› 1rdc

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURES OF RIBONUCLEASE HI ACTIVE SITE MUTANTS FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1rdc/pdb
PDB entry 1rdc coloured by chain, front view.
PDB entry 1rdc coloured by chain, side view.
PDB entry 1rdc coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of ribonuclease HI active site mutants from Escherichia coli.
Katayanagi K, Ishikawa M, Okumura M, Ariyoshi M, Kanaya S, Kawano Y, Suzuki M, Tanaka I, Morikawa K
J Biol Chem 268 22092-9 (1993)
PMID: 8408067

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HI Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.62 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A7Y4 (Residues: 1-155; Coverage: 100%)
Gene names: JW0204, b0214, dasF, herA, rnh, rnhA, sdrA
Sequence domains: RNase H
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 42.01Å b: 86.42Å c: 35.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 not available not available
Expression system: Not provided

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Citations

1 review citation

Ribonuclease H evolution in retrotransposable elements.
Malik HS. (2005)
13 other articles