› 1v8b

X-ray diffraction
2.4Å resolution

Crystal structure of a hydrolase

Released:
DOI: 10.2210/pdb1v8b/pdb
PDB entry 1v8b coloured by chain, front view.
PDB entry 1v8b coloured by chain, side view.
PDB entry 1v8b coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Crystal structure of S-adenosyl-L-homocysteine hydrolase from the human malaria parasite Plasmodium falciparum.
Tanaka N, Nakanishi M, Kusakabe Y, Shiraiwa K, Yabe S, Ito Y, Kitade Y, Nakamura KT
J Mol Biol 343 1007-17 (2004)
PMID: 15476817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 479 amino acids
Theoretical weight: 53.9 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: P50250 (Residues: 1-479; Coverage: 100%)
Gene name: PFE1050w
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAD 4 x NAD
Ligand ADN 4 x ADN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 77.09Å b: 86.15Å c: 333.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.24
Expression system: Escherichia coli

Quick links

Citations

7 review citations

S-adenosylmethionine and its products.
Grillo et al. (2008)
6 more

17 mentions without citation

S-adenosyl-L-homocysteine hydrolase and methylation disorders: yeast as a model system.
Tehlivets et al. (2013)
16 more