› 1wsh

X-ray diffraction
1.9Å resolution

Crystal structure of E.coli RNase HI active site mutant (E48A/K87A)

Released:
DOI: 10.2210/pdb1wsh/pdb
PDB entry 1wsh coloured by chain, front view.
PDB entry 1wsh coloured by chain, side view.
PDB entry 1wsh coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Tsunaka Y, Takano K, Matsumura H, Yamagata Y, Kanaya S

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 155 amino acids
Theoretical weight: 17.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Y4 (Residues: 1-155; Coverage: 100%)
Gene names: JW0204, b0214, dasF, herA, rnh, rnhA, sdrA
Sequence domains: RNase H
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21212
Unit cell:
a: 120.931Å b: 79.92Å c: 64.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.26
Expression system: Escherichia coli

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