› 1zjz

X-ray diffraction
1.1Å resolution

Structure of R-specific alcohol dehydrogenase (mutant G37D) from Lactobacillus brevis in complex with phenylethanol and NAD

Released:
DOI: 10.2210/pdb1zjz/pdb
PDB entry 1zjz coloured by chain, front view.
PDB entry 1zjz coloured by chain, side view.
PDB entry 1zjz coloured by chain, top view.
Source organism: Levilactobacillus brevis
Primary publication:
Atomic resolution structures of R-specific alcohol dehydrogenase from Lactobacillus brevis provide the structural bases of its substrate and cosubstrate specificity.
Schlieben NH, Niefind K, Müller J, Riebel B, Hummel W, Schomburg D
J Mol Biol 349 801-13 (2005)
PMID: 15896805

Function and Biology Details

Reaction catalysed: 
An alcohol + NADP(+) = an aldehyde + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
R-specific alcohol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 26.71 KDa
Source organism: Levilactobacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84EX5 (Residues: 2-252; Coverage: 100%)
Gene name: radh
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
Ligand MG 1 x MG
Ligand SS2 1 x SS2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 55.856Å b: 79.847Å c: 112.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.124 0.138
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Asymmetric bioreduction of activated C=C bonds using enoate reductases from the old yellow enzyme family.
Stuermer et al. (2007)
5 more

2 mentions without citation

Atomic-resolution structures of horse liver alcohol dehydrogenase with NAD(+) and fluoroalcohols define strained Michaelis complexes.
Plapp et al. (2012)
1 more