› 2khw

Solution NMR

Solution Structure of the human Polymerase iota UBM2-Ubiquitin Complex

Released:
DOI: 10.2210/pdb2khw/pdb
PDB entry 2khw coloured by chain, ensemble of 25 models, front view.
PDB entry 2khw coloured by chain, ensemble of 25 models, side view.
PDB entry 2khw coloured by chain, ensemble of 25 models, top view.
Source organisms:
Entry authors: Bomar MG, D'Souza S, Bienko M, Dikic I, Walker G

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143246 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase iota; Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.35 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P19909 (Residues: 304-359; Coverage: 10%)
  • Canonical: Q9UNA4 (Residues: 699-740; Coverage: 6%)
Gene names: POLI, RAD30B, spg
Sequence domains:
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 79 amino acids
Theoretical weight: 8.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

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