› 2pha

X-ray diffraction
1.9Å resolution

Crystal structure of native, unliganded human arginase at 1.90 resolution

Released:
DOI: 10.2210/pdb2pha/pdb
PDB entry 2pha coloured by chain, front view.
PDB entry 2pha coloured by chain, side view.
PDB entry 2pha coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human arginase I complexed with thiosemicarbazide reveals an unusual thiocarbonyl mu-sulfide ligand in the binuclear manganese cluster.
Di Costanzo L, Pique ME, Christianson DW
J Am Chem Soc 129 6388-9 (2007)
PMID: 17469833

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 34.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05089 (Residues: 1-322; Coverage: 100%)
Gene name: ARG1
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P3
Unit cell:
a: 90.77Å b: 90.77Å c: 69.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.244
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Physiological implications of arginine metabolism in plants.
Winter et al. (2015)
6 more

6 mentions without citation

Crystal structure of an arginase-like protein from Trypanosoma brucei that evolved without a binuclear manganese cluster.
Hai et al. (2015)
5 more