› 2cdq

X-ray diffraction
2.85Å resolution

Crystal structure of Arabidopsis thaliana aspartate kinase complexed with lysine and S- adenosylmethionine

Released:
DOI: 10.2210/pdb2cdq/pdb
PDB entry 2cdq coloured by chain, front view.
PDB entry 2cdq coloured by chain, side view.
PDB entry 2cdq coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
A novel organization of ACT domains in allosteric enzymes revealed by the crystal structure of Arabidopsis aspartate kinase.
Mas-Droux C, Curien G, Robert-Genthon M, Laurencin M, Ferrer JL, Dumas R
Plant Cell 18 1681-92 (2006)
PMID: 16731588

Function and Biology Details

Reaction catalysed: 
ATP + L-aspartate = ADP + 4-phospho-L-aspartate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartokinase 1, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 510 amino acids
Theoretical weight: 55.96 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9LYU8 (Residues: 61-569; Coverage: 90%)
Gene names: AK, AK-LYS1, AK1, At5g13280, T31B5.100
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand LYS 4 x LYS
Ligand TAR 2 x TAR
Ligand SAM 2 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: I41
Unit cell:
a: 117.342Å b: 117.342Å c: 255.283Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The Regulation of Essential Amino Acid Synthesis and Accumulation in Plants.
Galili et al. (2016)
3 more

7 mentions without citation

Research progress on unique paratope structure, antigen binding modes, and systematic mutagenesis strategies of single-domain antibodies.
Liu et al. (2022)
6 more