› 2f8z

X-ray diffraction
2.6Å resolution

Crystal structure of human FPPS in complex with zoledronate and isopentenyl diphosphate

Released:
DOI: 10.2210/pdb2f8z/pdb
PDB entry 2f8z coloured by chain, front view.
PDB entry 2f8z coloured by chain, side view.
PDB entry 2f8z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the exceptional in vivo efficacy of bisphosphonate drugs.
Rondeau JM, Bitsch F, Bourgier E, Geiser M, Hemmig R, Kroemer M, Lehmann S, Ramage P, Rieffel S, Strauss A, Green JR, Jahnke W
ChemMedChem 1 267-73 (2006)
PMID: 16892359

Function and Biology Details

Reactions catalysed: 
Prenyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Farnesyl pyrophosphate synthase Chain: F
Molecule details ›
Chain: F
Length: 350 amino acids
Theoretical weight: 40.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14324 (Residues: 72-419; Coverage: 83%)
Gene names: FDPS, FPS, KIAA1293
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand IPE 1 x IPE
Ligand ZOL 1 x ZOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 112.155Å b: 112.155Å c: 65.725Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.22 0.28
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Mechanisms of action of bisphosphonates: similarities and differences and their potential influence on clinical efficacy.
Russell et al. (2008)
17 more

11 mentions without citation

Computational insights into binding of bisphosphates to farnesyl pyrophosphate synthase.
Ohno et al. (2011)
10 more