› 2hs6

X-ray diffraction
1.9Å resolution

Crystal structure of the E291K mutant of 12-oxophytodienoate reductase 3 (OPR3) from tomato

Released:
DOI: 10.2210/pdb2hs6/pdb
PDB entry 2hs6 coloured by chain, front view.
PDB entry 2hs6 coloured by chain, side view.
PDB entry 2hs6 coloured by chain, top view.
Source organism: Solanum lycopersicum
Primary publication:
Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization.
Breithaupt C, Kurzbauer R, Lilie H, Schaller A, Strassner J, Huber R, Macheroux P, Clausen T
Proc Natl Acad Sci U S A 103 14337-42 (2006)
PMID: 16983071

Function and Biology Details

Reaction catalysed: 
8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate + NADP(+) = (15Z)-12-oxophyto-10,15-dienoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
12-oxophytodienoate reductase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 402 amino acids
Theoretical weight: 44.4 KDa
Source organism: Solanum lycopersicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FEW9 (Residues: 1-396; Coverage: 100%)
Gene name: OPR3
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 49.18Å b: 92.24Å c: 90.09Å
α: 90° β: 99.44° γ: 90°
R-values:
R R work R free
0.205 0.205 0.235
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany.
Wasternack et al. (2013)
8 more

1 mention without citation

Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization.
Breithaupt et al. (2006)