› 2i58

X-ray diffraction
2.8Å resolution

Crystal Structure of RafE from Streptococcus pneumoniae complexed with raffinose

Released:
DOI: 10.2210/pdb2i58/pdb
PDB entry 2i58 coloured by chain, front view.
PDB entry 2i58 coloured by chain, side view.
PDB entry 2i58 coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Entry authors: Paterson NG, Riboldi-Tunnicliffe A, Mitchell TJ, Isaacs NW

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sugar ABC transporter, sugar-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.45 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2URJ7 (Residues: 33-415; Coverage: 91%)
Gene names: SP_1897, rafE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLA, GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 49.214Å b: 119.435Å c: 146.326Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.211 0.296
Expression system: Escherichia coli

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