› 2i5n

X-ray diffraction
1.96Å resolution

1.96 A X-ray structure of photosynthetic reaction center from Rhodopseudomonas viridis:Crystals grown by microfluidic technique

Released:
DOI: 10.2210/pdb2i5n/pdb
Model geometry
Fit model/data
PDB entry 2i5n coloured by chain, front view.
PDB entry 2i5n coloured by chain, side view.
PDB entry 2i5n coloured by chain, top view.
Source organism: Blastochloris viridis
Primary publication:
Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins.
Li L, Mustafi D, Fu Q, Tereshko V, Chen DL, Tice JD, Ismagilov RF
Proc Natl Acad Sci U S A 103 19243-8 (2006)
PMID: 17159147

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-138851 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Photosynthetic reaction center cytochrome c subunit Chain: C
Molecule details ›
Chain: C
Length: 336 amino acids
Theoretical weight: 37.45 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P07173 (Residues: 21-356; Coverage: 100%)
Gene names: BVIRIDIS_00500, cytC, pufC
Sequence domains: Photosynthetic reaction centre cytochrome C subunit
Structure domains: Photosynthetic Reaction Center, subunit C, domain 2
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 258 amino acids
Theoretical weight: 28.56 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06008 (Residues: 1-258; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Reaction center protein M chain Chain: M

Ligands and Environments

11 bound ligands:
Ligand SO4 19 x SO4
Ligand HEC 4 x HEC
Ligand HTO 5 x HTO
Ligand LDA 4 x LDA
Ligand UNL 6 x UNL
Ligand FE2 1 x FE2
Ligand BCB 4 x BCB
Ligand BPB 2 x BPB
Ligand MQ9 1 x MQ9
Ligand UQ1 2 x UQ1
Ligand NS5 1 x NS5
1 modified residue:
Ligand FME 1 x FME

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P43212
Unit cell:
a: 220.4Å b: 220.4Å c: 113.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.19

Quick links

Citations

22 review citations

Droplet microfluidics.
Teh et al. (2008)
21 more

10 mentions without citation

Role of hydrogen-bond networks on the donor side of photosynthetic reaction centers from purple bacteria.
Fufina et al. (2023)
9 more