› 2iv2

X-ray diffraction
2.27Å resolution

Reinterpretation of reduced form of formate dehydrogenase H from E. coli

Released:
DOI: 10.2210/pdb2iv2/pdb
PDB entry 2iv2 coloured by chain, front view.
PDB entry 2iv2 coloured by chain, side view.
PDB entry 2iv2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Formate-reduced E. coli formate dehydrogenase H: The reinterpretation of the crystal structure suggests a new reaction mechanism.
Raaijmakers HC, Romão MJ
J Biol Inorg Chem 11 849-54 (2006)
PMID: 16830149

Function and Biology Details

Reaction catalysed: 
Formate + an [oxidized hydrogenase] = CO(2) + a [reduced hydrogenase]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase H Chain: X
Molecule details ›
Chain: X
Length: 715 amino acids
Theoretical weight: 79.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07658 (Residues: 1-715; Coverage: 100%)
Gene names: JW4040, b4079, fdhF
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SF4 1 x SF4
Ligand 2MD 1 x 2MD
Ligand MGD 1 x MGD
Ligand MO 1 x MO
Ligand UNX 1 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 146.4Å b: 146.4Å c: 81.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.226
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.
Ragsdale et al. (2008)
19 more

5 mentions without citation

Metabolically engineered bacteria for producing hydrogen via fermentation.
Vardar-Schara et al. (2008)
4 more