2q3r

X-ray diffraction
2Å resolution

Ensemble refinement of the protein crystal structure of At1g76680 from Arabidopsis thaliana

Released:

Function and Biology Details

Reaction catalysed:
8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate + NADP(+) = (15Z)-12-oxophyto-10,15-dienoate + NADPH
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-185273 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
12-oxophytodienoate reductase 1 Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 41.22 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8LAH7 (Residues: 1-372; Coverage: 100%)
Gene names: At1g76680, F28O16.5, OPR1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 46.688Å b: 88.066Å c: 149.334Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.282
Expression system: Escherichia coli