› 2r58

X-ray diffraction
2Å resolution

Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in Complex with Di-Methyl Lysine

Released:
DOI: 10.2210/pdb2r58/pdb
PDB entry 2r58 coloured by chain, front view.
PDB entry 2r58 coloured by chain, side view.
PDB entry 2r58 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural and functional analyses of methyl-lysine binding by the malignant brain tumour repeat protein Sex comb on midleg.
Grimm C, de Ayala Alonso AG, Rybin V, Steuerwald U, Ly-Hartig N, Fischle W, Müller J, Müller CW
EMBO Rep 8 1031-7 (2007)
PMID: 17932512

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polycomb protein Scm Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.4 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VHA0 (Residues: 175-435; Coverage: 30%)
Gene names: CG9495, Scm
Sequence domains: mbt repeat
Structure domains: SH3 type barrels.

Ligands and Environments

1 bound ligand:
Ligand MLY 1 x MLY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 83.12Å b: 83.12Å c: 75.768Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.229
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Histones: annotating chromatin.
Campos et al. (2009)
9 more

2 mentions without citation

Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila.
McElroy et al. (2014)
1 more