› 2reh

X-ray diffraction
2.4Å resolution

Mechanistic and Structural Analyses of the Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase

Released:
DOI: 10.2210/pdb2reh/pdb
PDB entry 2reh coloured by chain, front view.
PDB entry 2reh coloured by chain, side view.
PDB entry 2reh coloured by chain, top view.
Source organism: Fusarium oxysporum
Primary publication:
Mechanistic and structural analyses of the roles of Arg409 and Asp402 in the reaction of the flavoprotein nitroalkane oxidase.
Fitzpatrick PF, Bozinovski DM, Héroux A, Shaw PG, Valley MP, Orville AM
Biochemistry 46 13800-8 (2007)
PMID: 17994768

Function and Biology Details

Reaction catalysed: 
A nitroalkane + H(2)O + O(2) = an aldehyde or ketone + nitrite + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitroalkane oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 439 amino acids
Theoretical weight: 48.23 KDa
Source organism: Fusarium oxysporum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X1D8 (Residues: 1-439; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 108.934Å b: 108.934Å c: 337.671Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.204 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Nitronate monooxygenase, a model for anionic flavin semiquinone intermediates in oxidative catalysis.
Gadda et al. (2010)
1 more

2 mentions without citation

Mechanistic and structural analyses of the roles of Arg409 and Asp402 in the reaction of the flavoprotein nitroalkane oxidase.
Fitzpatrick et al. (2007)
1 more