› 2ux5

X-ray diffraction
2.21Å resolution

X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 9 in the charge-separated state

Released:
DOI: 10.2210/pdb2ux5/pdb
PDB entry 2ux5 coloured by chain, front view.
PDB entry 2ux5 coloured by chain, side view.
PDB entry 2ux5 coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
pH modulates the quinone position in the photosynthetic reaction center from Rhodobacter sphaeroides in the neutral and charge separated states.
Koepke J, Krammer EM, Klingen AR, Sebban P, Ullmann GM, Fritzsch G
J Mol Biol 371 396-409 (2007)
PMID: 17570397

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-533422 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 307 amino acids
Theoretical weight: 34.4 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like

Ligands and Environments

11 bound ligands:
Ligand GOL 7 x GOL
Ligand BCL 4 x BCL
Ligand LDA 9 x LDA
Ligand BPH 2 x BPH
Ligand UQ2 1 x UQ2
Ligand PO4 1 x PO4
Ligand HTO 1 x HTO
Ligand FE 1 x FE
Ligand U10 1 x U10
Ligand SPO 1 x SPO
Ligand CDL 1 x CDL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P3121
Unit cell:
a: 139.46Å b: 139.46Å c: 184.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.202 0.236

Quick links

Citations

7 review citations

Quantum design of photosynthesis for bio-inspired solar-energy conversion.
Romero et al. (2017)
6 more

1 mention without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)