› 2v7g

X-ray diffraction
2Å resolution

Crystal Structure of an Engineered Urocanase Tetramer

Released:
DOI: 10.2210/pdb2v7g/pdb
PDB entry 2v7g coloured by chain, front view.
PDB entry 2v7g coloured by chain, side view.
PDB entry 2v7g coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Designed protein-protein association.
Grueninger D, Treiber N, Ziegler MO, Koetter JW, Schulze MS, Schulz GE
Science 319 206-9 (2008)
PMID: 18187656

Function and Biology Details

Reaction catalysed: 
3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urocanate hydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 557 amino acids
Theoretical weight: 60.81 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25080 (Residues: 1-557; Coverage: 100%)
Gene name: hutU
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand ACT 7 x ACT
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 68.068Å b: 154.316Å c: 216.391Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.156 0.181
Expression system: Escherichia coli BL21(DE3)

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Citations

20 review citations

Caught in self-interaction: evolutionary and functional mechanisms of protein homooligomerization.
Hashimoto et al. (2011)
19 more