› 2zxq

X-ray diffraction
2Å resolution

Crystal structure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF)

Released:
DOI: 10.2210/pdb2zxq/pdb
PDB entry 2zxq coloured by chain, front view.
PDB entry 2zxq coloured by chain, side view.
PDB entry 2zxq coloured by chain, top view.
Source organism: Bifidobacterium longum
Primary publication:
Crystallographic and mutational analyses of substrate recognition of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum.
Suzuki R, Katayama T, Kitaoka M, Kumagai H, Wakagi T, Shoun H, Ashida H, Yamamoto K, Fushinobu S
J Biochem 146 389-98 (2009)
PMID: 19502354

Function and Biology Details

Reaction catalysed: 
Beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-L-serine/L-threonine-[protein] + H(2)O = beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosamine + L-serine/L-threonine-[protein]
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P65
Unit cell:
a: 192.267Å b: 192.267Å c: 123.017Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.172 0.196
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Mucin glycan foraging in the human gut microbiome.
Tailford et al. (2015)
5 more

4 mentions without citation

Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes.
Fushinobu et al. (2021)
3 more