› 3fq9

X-ray diffraction
1.35Å resolution

Design of an insulin analog with enhanced receptor-binding selectivity. Rationale, structure, and therapeutic implications

Released:
DOI: 10.2210/pdb3fq9/pdb
PDB entry 3fq9 coloured by chain, front view.
PDB entry 3fq9 coloured by chain, side view.
PDB entry 3fq9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Design of an insulin analog with enhanced receptor binding selectivity: rationale, structure, and therapeutic implications.
Zhao M, Wan ZL, Whittaker L, Xu B, Phillips NB, Katsoyannis PG, Ismail-Beigi F, Whittaker J, Weiss MA
J Biol Chem 284 32178-87 (2009)
PMID: 19773552

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 91-110; Coverage: 23%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
2 modified residues:
Ligand DAL 2 x DAL
Ligand DAB 2 x DAB

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: R3
Unit cell:
a: 81.841Å b: 81.841Å c: 33.518Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.228
Expression system: Not provided

Quick links

Citations

1 review citation

Improving drug-like properties of insulin and GLP-1 via molecule design and formulation and improving diabetes management with device & drug delivery.
Pechenov et al. (2017)
5 other articles

2 mentions without citation

Insulin analog with additional disulfide bond has increased stability and preserved activity.
Vinther et al. (2013)
1 more