› 3w5j

X-ray diffraction
1.93Å resolution

Crystal structure of GDP-bound NfeoB from Gallionella capsiferriformans

Released:
DOI: 10.2210/pdb3w5j/pdb
PDB entry 3w5j coloured by chain, front view.
PDB entry 3w5j coloured by chain, side view.
PDB entry 3w5j coloured by chain, top view.
Source organism: Gallionella capsiferriformans ES-2
Primary publication:
Structure of an atypical FeoB G-domain reveals a putative domain-swapped dimer.
Deshpande CN, McGrath AP, Font J, Guilfoyle AP, Maher MJ, Jormakka M
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 399-404 (2013)
PMID: 23545645

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferrous iron transport protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 22.73 KDa
Source organism: Gallionella capsiferriformans ES-2
Expression system: Escherichia coli
UniProt:
  • Canonical: D9SIP4 (Residues: 2-202; Coverage: 34%)
Gene name: Galf_2203
Sequence domains: Ferrous iron transport protein B
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand GOL 1 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 45.76Å b: 84.04Å c: 95.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.232
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial ferrous iron transport: the Feo system.
Lau et al. (2016)
7 other articles

2 mentions without citation

Ins and Outs: Recent Advancements in Membrane Protein-Mediated Prokaryotic Ferrous Iron Transport.
Brown et al. (2021)
1 more