› 3aoa

X-ray diffraction
3.35Å resolution

Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket

Released:
DOI: 10.2210/pdb3aoa/pdb
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PDB entry 3aoa coloured by chain, front view.
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PDB entry 3aoa coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket.
Nakashima R, Sakurai K, Yamasaki S, Nishino K, Yamaguchi A
Nature 480 565-9 (2011)
PMID: 22121023

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1053 amino acids
Theoretical weight: 114.22 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 226.533Å b: 134.438Å c: 162.783Å
α: 90° β: 97.73° γ: 90°
R-values:
R R work R free
0.274 0.271 0.326
Expression system: Escherichia coli

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Citations

44 review citations

Molecular mechanisms of antibiotic resistance.
Blair et al. (2015)
43 more

5 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
4 more

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