› 3atz

X-ray diffraction
2.04Å resolution

Crystal structure of TcOYE with pHBA

Released:
DOI: 10.2210/pdb3atz/pdb
PDB entry 3atz coloured by chain, front view.
PDB entry 3atz coloured by chain, side view.
PDB entry 3atz coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Structural insight into the stereoselective production of PGF2α by Old Yellow Enzyme from Trypanosoma cruzi.
Okamoto N, Yamaguchi K, Mizohata E, Tokuoka K, Uchiyama N, Sugiyama S, Matsumura H, Inaka K, Urade Y, Inoue T
J Biochem 150 563-8 (2011)
PMID: 21840922

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 379 amino acids
Theoretical weight: 42.31 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I6L9 (Residues: 1-379; Coverage: 100%)
Gene names: ECC02_012910, ECC02_013670, TcPGFS
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand FMN 4 x FMN
Ligand HBA 4 x HBA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 47.333Å b: 119.587Å c: 111.527Å
α: 90° β: 90.35° γ: 90°
R-values:
R R work R free
0.172 0.172 0.219
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Old yellow enzymes: structures and structure-guided engineering for stereocomplementary bioreduction.
Shi et al. (2020)
4 other articles

3 mentions without citation

Enzymes of the cyclooxygenase pathways of prostanoid biosynthesis.
Smith et al. (2011)
2 more