› 3b3r

X-ray diffraction
0.98Å resolution

Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)

Released:
DOI: 10.2210/pdb3b3r/pdb
PDB entry 3b3r coloured by chain, front view.
PDB entry 3b3r coloured by chain, side view.
PDB entry 3b3r coloured by chain, top view.
Source organism: Streptomyces sp. SA-COO
Primary publication:
Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase.
Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A
Protein Sci 16 2647-56 (2007)
PMID: 18029419

Function and Biology Details

Reactions catalysed: 
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 506 amino acids
Theoretical weight: 55.12 KDa
Source organism: Streptomyces sp. SA-COO
Expression system: Escherichia coli
UniProt:
  • Canonical: P12676 (Residues: 42-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAE 1 x FAE
2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 51.314Å b: 73.643Å c: 63.275Å
α: 90° β: 104.98° γ: 90°
R-values:
R R work R free
0.129 not available 0.159
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Cholesterol oxidase: biochemistry and structural features.
Vrielink et al. (2009)
1 more

2 mentions without citation

Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase.
Lyubimov et al. (2007)
1 more