Function and Biology Details
Reactions catalysed:
Toluene + NADH + O(2) = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD(+)
Benzene + NADH + O(2) = cis-cyclohexa-3,5-diene-1,2-diol + NAD(+)
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Aromatic-ring-hydroxylating dioxygenase, alpha subunit
- Ring-hydroxylating dioxygenase beta subunit
- Rieske [2Fe-2S] iron-sulphur domain
- Rieske [2Fe-2S] iron-sulphur domain superfamily
- NTF2-like domain superfamily
- Ring-hydroxylating dioxygenase, alpha subunit NdoB-like, C-terminal
- Aromatic-ring-hydroxylating dioxygenase, alpha subunit, C-terminal domain
- Aromatic-ring-hydroxylating dioxygenase, 2Fe-2S-binding site
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
Assembly name:
Benzene 1,2-dioxygenase (preferred)
PDBe Complex ID:
PDB-CPX-107772 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Benzene 1,2-dioxygenase subunit alpha
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 51 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains:
Length: 450 amino acids
Theoretical weight: 51 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
- Canonical:
A5W4F2 (Residues: 1-450; Coverage: 100%)
Sequence domains:
Structure domains:
Benzene 1,2-dioxygenase subunit beta
Molecule details ›
Chain: B
Length: 187 amino acids
Theoretical weight: 22.04 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
Sequence domains: Ring hydroxylating beta subunit
Structure domains: Nuclear Transport Factor 2; Chain: A,
Length: 187 amino acids
Theoretical weight: 22.04 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
- Canonical: A5W4F1 (Residues: 1-187; Coverage: 100%)
Sequence domains: Ring hydroxylating beta subunit
Structure domains: Nuclear Transport Factor 2; Chain: A,
