› 3gr7

X-ray diffraction
2.3Å resolution

Structure of OYE from Geobacillus kaustophilus, hexagonal crystal form

Released:
DOI: 10.2210/pdb3gr7/pdb
PDB entry 3gr7 coloured by chain, front view.
PDB entry 3gr7 coloured by chain, side view.
PDB entry 3gr7 coloured by chain, top view.
Source organism: Geobacillus kaustophilus
Primary publication:
Old Yellow Enzyme-Catalyzed Dehydrogenation of Saturated Ketones
Schittmayer M, Zach S, Winkler M, Winkler A, Macheroux P, Uhl MK, Gruber K, Kambourakis S, Rozzell D, Glieder A
Adv. Synth. Catal. 353 268-274 (2011)

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177376 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 37.73 KDa
Source organism: Geobacillus kaustophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KXG9 (Residues: 1-340; Coverage: 100%)
Gene names: GK2332, namA
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P6522
Unit cell:
a: 101.99Å b: 101.99Å c: 260.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.223
Expression system: Escherichia coli

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