› 3gx9

X-ray diffraction
2.28Å resolution

Structure of morphinone reductase N189A mutant in complex with tetrahydroNAD

Released:
DOI: 10.2210/pdb3gx9/pdb
PDB entry 3gx9 coloured by chain, front view.
PDB entry 3gx9 coloured by chain, side view.
PDB entry 3gx9 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Parallel pathways and free-energy landscapes for enzymatic hydride transfer probed by hydrostatic pressure.
Pudney CR, McGrory T, Lafite P, Pang J, Hay S, Leys D, Sutcliffe MJ, Scrutton NS
Chembiochem 10 1379-84 (2009)
PMID: 19405065

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 41.25 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51990 (Residues: 1-377; Coverage: 100%)
Gene name: morB
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand FMN 1 x FMN
Ligand TXD 1 x TXD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I212121
Unit cell:
a: 49.651Å b: 122.941Å c: 179.587Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.229
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Gating mechanisms for biological electron transfer: integrating structure with biophysics reveals the nature of redox control in cytochrome P450 reductase and copper-dependent nitrite reductase.
Leferink et al. (2012)
5 more