› 3h9u

X-ray diffraction
1.9Å resolution

S-adenosyl homocysteine hydrolase (SAHH) from Trypanosoma brucei

Released:
DOI: 10.2210/pdb3h9u/pdb
PDB entry 3h9u coloured by chain, front view.
PDB entry 3h9u coloured by chain, side view.
PDB entry 3h9u coloured by chain, top view.
Source organism: Trypanosoma brucei
Entry authors: Siponen MI, Schutz P, Arrowsmith CH, Berglund H, Bountra C, Collins R, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson A, Johansson I, Karlberg T, Kragh Nielsen T, Kotenyova T, Kotzsch A, Moche M, Nordlund P, Nyman T, Persson C, Sagemark J, Thorsell AG, Tresaugues L, Van Den Berg S, Weigelt J, Welin M, Wisniewska M, Schueler H, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo octamer
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 436 amino acids
Theoretical weight: 48.38 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q383X0 (Residues: 4-437; Coverage: 99%)
Gene name: Tb11.01.1350
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NAD 4 x NAD
Ligand ADN 4 x ADN
Ligand GOL 1 x GOL
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 91.35Å b: 136.05Å c: 191.87Å
α: 90° β: 99.32° γ: 90°
R-values:
R R work R free
0.198 0.196 0.231
Expression system: Escherichia coli

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