› 3hgs

X-ray diffraction
2Å resolution

Crystal structure of tomato OPR3 in complex with pHB

Released:
DOI: 10.2210/pdb3hgs/pdb
PDB entry 3hgs coloured by chain, front view.
PDB entry 3hgs coloured by chain, side view.
PDB entry 3hgs coloured by chain, top view.
Source organism: Solanum lycopersicum
Primary publication:
Structural basis of substrate specificity of plant 12-oxophytodienoate reductases.
Breithaupt C, Kurzbauer R, Schaller F, Stintzi A, Schaller A, Huber R, Macheroux P, Clausen T
J Mol Biol 392 1266-77 (2009)
PMID: 19660473

Function and Biology Details

Reaction catalysed: 
8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate + NADP(+) = (15Z)-12-oxophyto-10,15-dienoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
12-oxophytodienoate reductase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 402 amino acids
Theoretical weight: 44.4 KDa
Source organism: Solanum lycopersicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FEW9 (Residues: 1-396; Coverage: 100%)
Gene name: OPR3
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand PHB 2 x PHB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 57.61Å b: 89.5Å c: 80.68Å
α: 90° β: 107.94° γ: 90°
R-values:
R R work R free
0.212 0.206 0.236
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Enzymes in jasmonate biosynthesis - structure, function, regulation.
Schaller et al. (2009)
4 more