› 3hqp

X-ray diffraction
2.3Å resolution

Crystal structure of Leishmania mexicana pyruvate kinase (LmPYK) in complex with ATP, Oxalate and fructose 2,6 bisphosphate

Released:
DOI: 10.2210/pdb3hqp/pdb
PDB entry 3hqp coloured by chain, front view.
PDB entry 3hqp coloured by chain, side view.
PDB entry 3hqp coloured by chain, top view.
Source organism: Leishmania mexicana
Primary publication:
Allosteric mechanism of pyruvate kinase from Leishmania mexicana uses a rock and lock model.
Morgan HP, McNae IW, Nowicki MW, Hannaert V, Michels PA, Fothergill-Gilmore LA, Walkinshaw MD
J Biol Chem 285 12892-8 (2010)
PMID: 20123988

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 499 amino acids
Theoretical weight: 54.47 KDa
Source organism: Leishmania mexicana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27686 (Residues: 1-499; Coverage: 100%)
Gene name: PYK
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand MG 30 x MG
Ligand K 32 x K
Ligand OXL 15 x OXL
Ligand FDP 16 x FDP
Ligand ATP 15 x ATP
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 127.486Å b: 151.14Å c: 160.319Å
α: 89.73° β: 80.17° γ: 71.64°
R-values:
R R work R free
0.201 0.198 0.252
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metalloproteins in the Biology of Heterocysts.
Pernil et al. (2019)
1 more

12 mentions without citation

An overview of structure, function, and regulation of pyruvate kinases.
Schormann et al. (2019)
11 more