› 3k30

X-ray diffraction
2.7Å resolution

Histamine dehydrogenase from Nocardiodes simplex

Released:
DOI: 10.2210/pdb3k30/pdb
PDB entry 3k30 coloured by chain, front view.
PDB entry 3k30 coloured by chain, side view.
PDB entry 3k30 coloured by chain, top view.
Source organism: Pimelobacter simplex
Primary publication:
Crystal structure of histamine dehydrogenase from Nocardioides simplex.
Reed T, Lushington GH, Xia Y, Hirakawa H, Travis DM, Mure M, Scott EE, Limburg J
J Biol Chem 285 25782-91 (2010)
PMID: 20538584

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histamine dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 690 amino acids
Theoretical weight: 75.66 KDa
Source organism: Pimelobacter simplex
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6IWJ5 (Residues: 1-690; Coverage: 100%)
Gene name: hdh
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SF4 2 x SF4
Ligand FMN 2 x FMN
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 101.141Å b: 107.035Å c: 153.352Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.242
Expression system: Escherichia coli

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Citations

1 review citation

Old yellow enzymes: structures and structure-guided engineering for stereocomplementary bioreduction.
Shi et al. (2020)
7 other articles

6 mentions without citation

The combined structural and kinetic characterization of a bacterial nitronate monooxygenase from Pseudomonas aeruginosa PAO1 establishes NMO class I and II.
Salvi et al. (2014)
5 more