› 3kzf

X-ray diffraction
3Å resolution

Structure of Giardia Carbamate Kinase

Released:
DOI: 10.2210/pdb3kzf/pdb
PDB entry 3kzf coloured by chain, front view.
PDB entry 3kzf coloured by chain, side view.
PDB entry 3kzf coloured by chain, top view.
Source organism: Giardia lamblia ATCC 50803
Primary publication:
X-ray structure and characterization of carbamate kinase from the human parasite Giardia lamblia.
Galkin A, Kulakova L, Wu R, Nash TE, Dunaway-Mariano D, Herzberg O
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 386-90 (2010)
PMID: 20383005

Function and Biology Details

Reaction catalysed: 
(1a) ATP + carbamate = ADP + carbamoyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbamate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 33.98 KDa
Source organism: Giardia lamblia ATCC 50803
Expression system: Escherichia coli
UniProt:
  • Canonical: A8BB85 (Residues: 1-316; Coverage: 100%)
Gene name: GL50803_0016453
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 69.77Å b: 85.41Å c: 102.1Å
α: 90° β: 106.8° γ: 90°
R-values:
R R work R free
0.227 0.227 0.283
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

A homogenous luminescence assay reveals novel inhibitors for giardia lamblia carbamate kinase.
Chen et al. (2012)
4 more

2 mentions without citation

Structural characterization of the enzymes composing the arginine deiminase pathway in Mycoplasma penetrans.
Gallego et al. (2012)
1 more