› 3lif

X-ray diffraction
2.7Å resolution

Crystal Structure of the extracellular domain of the putative histidine kinase rpHK1S-Z16

Released:
DOI: 10.2210/pdb3lif/pdb
PDB entry 3lif coloured by chain, front view.
PDB entry 3lif coloured by chain, side view.
PDB entry 3lif coloured by chain, top view.
Source organism: Rhodopseudomonas palustris
Primary publication:
Structural characterization of the predominant family of histidine kinase sensor domains.
Zhang Z, Hendrickson WA
J Mol Biol 400 335-53 (2010)
PMID: 20435045

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179950 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 254 amino acids
Theoretical weight: 28.77 KDa
Source organism: Rhodopseudomonas palustris
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6N3S7 (Residues: 40-293; Coverage: 39%)
Gene name: RPA3616
Sequence domains: Histidine kinase sensor domain
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand MPD 2 x MPD
Ligand CIT 2 x CIT
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P4332
Unit cell:
a: 184.78Å b: 184.78Å c: 184.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.256
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Evolution of two-component signal transduction systems.
Capra et al. (2012)
18 more

9 mentions without citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
8 more