› 3njn

X-ray diffraction
1.25Å resolution

Q118A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis

Released:
DOI: 10.2210/pdb3njn/pdb
PDB entry 3njn coloured by chain, front view.
PDB entry 3njn coloured by chain, side view.
PDB entry 3njn coloured by chain, top view.
Source organism: Shewanella oneidensis
Primary publication:
Characterization of member of DUF1888 protein family, self-cleaving and self-assembling endopeptidase.
Osipiuk J, Mulligan R, Bargassa M, Hamilton JE, Cunningham MA, Joachimiak A
J Biol Chem 287 19452-61 (2012)
PMID: 22493430

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dodecamer
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184383 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Autocatalytic aspartic peptidase Chains: A, C
Molecule details ›
Chains: A, C
Length: 119 amino acids
Theoretical weight: 12.66 KDa
Source organism: Shewanella oneidensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8EGA7 (Residues: 1-116; Coverage: 93%)
Gene name: SO_1698
Sequence domains: DP-EP family
Structure domains: Cupredoxins - blue copper proteins
Autocatalytic aspartic peptidase Chains: B, D
Molecule details ›
Chains: B, D
Length: 9 amino acids
Theoretical weight: 1.02 KDa
Source organism: Shewanella oneidensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8EGA7 (Residues: 117-125; Coverage: 7%)
Gene name: SO_1698

Ligands and Environments

2 bound ligands:
Ligand MPD 1 x MPD
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: R32
Unit cell:
a: 72.351Å b: 72.351Å c: 256.008Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.14 0.139 0.164
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Efficient expression of nattokinase in Bacillus licheniformis: host strain construction and signal peptide optimization.
Wei et al. (2015)
1 more

2 mentions without citation

Characterization of member of DUF1888 protein family, self-cleaving and self-assembling endopeptidase.
Osipiuk et al. (2012)
1 more