› 3oab

X-ray diffraction
2.3Å resolution

Mint deletion mutant of heterotetrameric geranyl pyrophosphate synthase in complex with ligands

Released:
DOI: 10.2210/pdb3oab/pdb
PDB entry 3oab coloured by chain, front view.
PDB entry 3oab coloured by chain, side view.
PDB entry 3oab coloured by chain, top view.
Source organism: Mentha x piperita
Primary publication:
Enhanced specificity of mint geranyl pyrophosphate synthase by modifying the R-loop interactions.
Hsieh FL, Chang TH, Ko TP, Wang AH
J Mol Biol 404 859-73 (2010)
PMID: 20965200

Function and Biology Details

Reaction catalysed: 
Prenyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-193440 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gpp synthase large subunit Chains: A, D
Molecule details ›
Chains: A, D
Length: 295 amino acids
Theoretical weight: 31.95 KDa
Source organism: Mentha x piperita
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SBR3 (Residues: 84-377; Coverage: 78%)
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase
Geranyl diphosphate synthase small subunit Chains: B, C
Molecule details ›
Chains: B, C
Length: 264 amino acids
Theoretical weight: 28.71 KDa
Source organism: Mentha x piperita
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SBR4 (Residues: 49-313; Coverage: 82%)
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

5 bound ligands:
Ligand PPV 1 x PPV
Ligand DST 2 x DST
Ligand MG 4 x MG
Ligand EDO 1 x EDO
Ligand IPE 1 x IPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 51.274Å b: 109.258Å c: 182.727Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.277
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structure and mechanism of an Arabidopsis medium/long-chain-length prenyl pyrophosphate synthase.
Hsieh et al. (2011)