› 3p4a

X-ray diffraction
1.2Å resolution

2'Fluoro modified RNA octamer fA2U2

Released:
DOI: 10.2210/pdb3p4a/pdb
PDB entry 3p4a coloured by chain, front view.
PDB entry 3p4a coloured by chain, side view.
PDB entry 3p4a coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Unexpected origins of the enhanced pairing affinity of 2'-fluoro-modified RNA.
Pallan PS, Greene EM, Jicman PA, Pandey RK, Manoharan M, Rozners E, Egli M
OpenAccess logo Nucleic Acids Res 39 3482-95 (2011)
PMID: 21183463

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196714 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
2'Fluoro modified RNA 8-MER Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 8 nucleotides
Theoretical weight: 2.54 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SR 9 x SR
Ligand MG 2 x MG
4 modified residues:
Ligand CFZ 12 x CFZ
Ligand GF2 12 x GF2
Ligand AF2 12 x AF2
Ligand UFT 12 x UFT

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3
Unit cell:
a: 43.245Å b: 43.245Å c: 60.791Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.17 0.234
Expression system: Not provided

Quick links

Citations

20 review citations

Designing chemically modified oligonucleotides for targeted gene silencing.
Deleavey et al. (2012)
19 more

8 mentions without citation

The structural diversity of artificial genetic polymers.
Anosova et al. (2016)
7 more