› 3qpb

X-ray diffraction
1.82Å resolution

Crystal Structure of Streptococcus Pyogenes Uridine Phosphorylase Reveals a Subclass of the NP-I Superfamily

Released:
DOI: 10.2210/pdb3qpb/pdb
PDB entry 3qpb coloured by chain, front view.
PDB entry 3qpb coloured by chain, side view.
PDB entry 3qpb coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M6
Primary publication:
The crystal structure of Streptococcus pyogenes uridine phosphorylase reveals a distinct subfamily of nucleoside phosphorylases.
Tran TH, Christoffersen S, Allan PW, Parker WB, Piskur J, Serra I, Terreni M, Ealick SE
Biochemistry 50 6549-58 (2011)
PMID: 21707079

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Length: 282 amino acids
Theoretical weight: 30.56 KDa
Source organism: Streptococcus pyogenes serotype M6
Expression system: Escherichia coli
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand R1P 18 x R1P
Ligand URA 18 x URA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 92.021Å b: 91.578Å c: 170.18Å
α: 78.46° β: 82.33° γ: 60.12°
R-values:
R R work R free
0.177 0.177 0.203
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Uridine phosphorylase from Trypanosoma cruzi: kinetic and chemical mechanisms.
Silva et al. (2011)
5 more

2 mentions without citation

The crystal structure of Streptococcus pyogenes uridine phosphorylase reveals a distinct subfamily of nucleoside phosphorylases.
Tran et al. (2011)
1 more