› 3qtg

X-ray diffraction
2.2Å resolution

Crystal structure of pyruvate kinase from Pyrobaculum aerophilum

Released:
DOI: 10.2210/pdb3qtg/pdb
PDB entry 3qtg coloured by chain, front view.
PDB entry 3qtg coloured by chain, side view.
PDB entry 3qtg coloured by chain, top view.
Source organism: Pyrobaculum aerophilum str. IM2
Entry authors: Davies C, Solomons JTG

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 461 amino acids
Theoretical weight: 50.32 KDa
Source organism: Pyrobaculum aerophilum str. IM2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8ZYE0 (Residues: 1-461; Coverage: 100%)
Gene names: PAE0819, pyk
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 116.3Å b: 107.4Å c: 105Å
α: 90° β: 110.5° γ: 90°
R-values:
R R work R free
0.208 0.206 0.247
Expression system: Escherichia coli BL21

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