› 3r48

X-ray diffraction
2Å resolution

Crystal structure of a hetero-hexamer coiled coil

Released:
DOI: 10.2210/pdb3r48/pdb
PDB entry 3r48 coloured by chain, front view.
PDB entry 3r48 coloured by chain, side view.
PDB entry 3r48 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
A de novo peptide hexamer with a mutable channel.
Zaccai NR, Chi B, Thomson AR, Boyle AL, Bartlett GJ, Bruning M, Linden N, Sessions RB, Booth PJ, Brady RL, Woolfson DN
OpenAccess logo Nat Chem Biol 7 935-41 (2011)
PMID: 22037471

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163966 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
coiled coil helix W22-L24H Chains: A, F, G
Molecule details ›
Chains: A, F, G
Length: 34 amino acids
Theoretical weight: 3.39 KDa
Source organism: synthetic construct
Expression system: Not provided
coiled coil helix Y15-L24D Chains: B, C, E
Molecule details ›
Chains: B, C, E
Length: 32 amino acids
Theoretical weight: 3.21 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 148.872Å b: 50.85Å c: 31.681Å
α: 90° β: 91.05° γ: 90°
R-values:
R R work R free
0.213 0.21 0.277
Expression system: Not provided

Quick links

Citations

24 review citations

The coming of age of de novo protein design.
Huang et al. (2016)
23 more

2 mentions without citation

Strategies to control the binding mode of de novo designed protein interactions.
Der et al. (2013)
1 more